figure3b

• Publication: Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
• Genotype
  • Strain: BL21-DE3
  • Plasmids: pSB1A2-pLasRV
• Environment
  • Medium: LB
  • Temperature: 37°C
  • Supplement: 3OC12HSL (mM)
• Phenotype
  • Measurement: GFPmut3b (au)
• Model
  • BBa_J23100 = 200*alpha_BBa_J23100
  • lasR = ALPHA_BBa_B0034*BBa_J23100
  • C_3OC12HSL_lasR = lasR*K_C_3OC12HSL^n_C_3OC12HSL/(K_C_3OC12HSL^n_C_3OC12HSL + C_3OC12HSL^n_C_3OC12HSL)
  • pLasRV = 200*(beta_pLasRV + (alpha_pLasRV - beta_pLasRV)*C_3OC12HSL_lasR^n_pLasRV/(K_pLasRV^n_pLasRV + C_3OC12HSL_lasR^n_pLasRV))
  • GFPmut3b = scale_GFPmut3b_doi_10_1093_nar_gkt052*ALPHA_BBa_B0034*pLasRV
• Data